ABSTRACT
Objective To develop a medical technical support module and box-type medical technical equipment so as to supply medical oxygen and water for the tent-form field medical system at field conditions.Methods The medical technical support module had its service orientation analyzed to determine its functional requirements and technical indexes, which involved in PSA oxygen generation technology with variable frequency,constant pressure and self-adaptability to altitudes as well as medical water preparation technology by integrated membrane separation. Box-type modules for medical oxygen generation and medial water preparation were trial-produced with the mode of integrated box and instruments as well as high-density polyethylene as the box material.Results The oxygen production was 1.3 Nm3/h,and the oxygen concentration was 92.7%.The flow volume of cleaning water and purified water was 433.7 and 37.2 L/h respectively at 25℃,and the water quality accorded with the relevant water quality indexes of GB 5749—2006 and the national pharmacopoeia(2010 edition). Conclusion This set of medical equipment can meet the design requirements and can effectively guarantee the medical oxygen and medical water needed for medical treatmentin field tent hospital under field conditions. [Chinese Medical Equipment Journal,2018,39(5):7-11]
ABSTRACT
OBJECTIVES@#To establish a novel method for the separation of sperm cells in mixed stain, and to evaluate its application value.@*METHODS@#Totally 40 mixed stain samples were collected from sexual assault cases. Sperm cells were separated by the conventional differential lysis method and the nylon membrane bushing separation technique, respectively. The DNA of sperm cells was extracted with the silicon membrane kit （Forensic DNA Extraction Kit for Soft Tissues）. The PCR amplification was performed using AmpFℓSTR® Identifiler® Plus kit, and the products were electrophoresed by 3500xL genetic analyser. The results of two separation methods were then compared.@*RESULTS@#Complete and single-source male STR genotypes could be obtained from all the 40 mixed stain samples except three samples with minimal residual of female DNA by the nylon membrane bushing separation technique. The STR genotypes of sperm cells could not be detected in 25 samples, which were obtained in 15 samples （seven were of incomplete male STR genotypes, six with residual of female DNA, two were complete and single-source STR genotypes of sperm cells）.@*CONCLUSIONS@#The nylon membrane bushing separation technique developed in present study can be used in the separation of sperm cells in mixed stain, especially for the extraction of a small amount of sperm from a large quantity of female cells, which is inexpensive, rapid and simple.
Subject(s)
Humans , Male , Coloring Agents , DNA/genetics , DNA Fingerprinting , Genotype , Microsatellite Repeats , Nylons , Polymerase Chain Reaction , Semen , Sex Offenses , SpermatozoaABSTRACT
Objective: To extract and purify thepolysaccharide from Portulacaoleracea and evaluate its anti-oxidant activity. Methods: Orthogonal experiment design L9(43) was employed to optimize the traditional extraction process. The processes of deproteinization and depigmentation were also investigated. Specifications of membrane with different apertures was used to purify the crude polysaccharide. The anti-oxidant activity of the polysaccharide was also evaluated by Fenton reaction and autoxidation of pyrogallol. Results: The polysaccharide was extracted at 100℃ for 3h with four times repeated to give the highest extraction rate. The recycling rates of Sevage method, thicloroacetic acid and hydrochloric acid method to remove protein were 17.05%, 9.66%, and 16.61%, respectively. The recycling rates of active carbon, H2O2, acetone-anhydrous ethanol, and chloroformn butanol to remove pigment were 17.04%, 17.09%, 27.06%, and 17.58%, respectively. Much of the polysaccharide were detected in the liquid penetrated from the membrane with apertures of 0.8μm.The clearance rate of polysaccharidescavenge hydroxyl radical and superoxide radicalwere 58.89%, and 38.89%. Conclusion: The preferred aperture of microfiltration and ultrafiltration membrane can purified P.oleracea. Polysaccharide antioxidant experiment indicates that the polysaccharide could effectively scavenge hydroxyl radical and superoxide radical with dose dependent relationship.